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Below is a list of the details of this product.
833.00
96 Tests
EKE62474
Below is a list of the details of this product.
NA
NA
NA
NA
Rabbit
PDGF-AA
Sandwich
4.5 hours
Ice packs
18.75 pg/ml
Colorimetric
31.25-2000 pg/ml
6-11 business days
Oryctolagus cuniculus
Serum, Plasma, Biological Fluids
Short term: 4°C; Long term: see manual.
For research use only. Not for diagnostic procedures.
The kit is manufactured at ISO 9001 certified facilities.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
No significant cross-reactivity or interference was observed.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Cancer, Cardiovascular, Metabolism, Immunology, Signal transduction
Use Rabbit PDGF-AA (Platelet Derived Growth Factor AA) ELISA Kit before 6 months
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Rabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit PDGF-AA . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PDGF-AA and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit PDGF-AA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit PDGF-AA. The concentration of Rabbit PDGF-AA in samples can be calculated by comparing the OD of the samples to the standard curve.
