We invite you to familiarize yourself with our list of PDGF-AA products.
Below is a list of the details of this product.
180.00
0.1 mg
XP-5254
Below is a list of the details of this product.
5154
Human
PDGFA
129719
Rabbit
P04085
P04085
anticorps
Polyclonal
Lyophilized
Homo sapiens
unlabelled serum
ELISA, Western Blot, NELISAut
platelet-derived growth factor alpha polypeptide
PDGF1, PDGF-A, PDGF1, Platelet-derived growth factor subunit A, PDGF-1, PDGF subunit A
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-AA.
Anti-hPDGF-AA specific antibody was purified by affinity chromatography employing immobilized hPDGF-AA matrix.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PDGF-AA antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (10.0 ng/mL), a concentration of 0.17 - 0.19 ug/mL of this antibody is required. ELISA:To detect hPDGF-AA by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich:To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PDGF-AA (XP-5254Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA.Western Blot:To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
