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Below is a list of the details of this product.
541.00
96Test
E-EL-RB1253-96T
Below is a list of the details of this product.
NA
NA
Rabbit
PDGF-AA
Sandwich
18.75pg/ml
Colormetric
31.25~2000pg/ml
Oryctolagus cuniculus
Serum, Plasma, Cell supernatant
Rabbit PDGF-AA(Platelet Derived Growth Factor AA) EIA Kit
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
Rabbits are used for polyclonal antibody production by Elabscience. Rabbit antibodies are very stable and can be stored for several days at room temperature. Elabscience adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rabbit PDGF-AA. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PDGF-AA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit PDGF-AA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit PDGF-AA. You can calculate the concentration of Rabbit PDGF-AA in the samples by comparing the OD of the samples to the standard curve.
